Site Directed Mutagenesis (SDM) Human Point mutation THP-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - THP-1

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - THP-1

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized THP-1

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media THP-1

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media THP-1

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1