Site Directed Mutagenesis (SDM) Mouse Point mutation 3T3-L1

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Mouse - NIH 3T3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse NIH 3T3

Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 G3BP

Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 FXR

Get tips on using FITC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using APC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

Get tips on using siGENOME Mouse Alox12 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 12-Lox/ALOX12