Site Directed Mutagenesis (SDM) Rat

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - RAW264.7

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - RAW264.7

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media RAOSMC

Get tips on using Anti-Ki-67 Antibody to perform Immunohistochemistry Ki67 - Rabbit Mouse -NA-

Get tips on using Low Molecular Weight DNA Ladder to perform DNA Ladder Low Range

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Rael

Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - immortalized Raji