siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - mouse cardiomyocytes

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using REPLI-g Advanced DNA Single Cell Kit (96) to perform Whole Genome Amplification Mouse

Get tips on using QIAseq FX Single Cell DNA Library Kit (96) to perform Whole Genome Amplification Parasites