siRNA / RNAi /miRNA transfection Human Cells OV-2008

- Found 9172 results

Get tips on using Type-it HRM PCR Kit (2000) to perform High-resolution melting (HRM) analysis Bacterial

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Get tips on using cyclin D1 Antibody (DCS-6): sc-20044 to perform Western blotting Cyclin D1

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Get tips on using miScript SYBR Green PCR Kit (2000) to perform PCR Quantitative real-time PCR - Mammalian DNA

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Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

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Get tips on using Ovation® RNA-Seq System V2 to perform RNA sequencing Human - H7

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Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - H9

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Reporter gene assays are designed to test the regulation of the expression of a gene of interest. This is usually done by linking the promoter of the gene of interest with a gene such as a firefly luciferase, which can be easily detected by addition of luciferin that leads to an enzymatic reaction to produce luminescence. The enzymatic reaction can be correlated to the expression of the gene of interest. Another luciferase gene that can be used is Renilla luciferase. For an appropriate luciferase assay: 1. the reporter should express uniformly in all cells, 2. specifically respond to effectors that the assay intends to monitor, 3. have low intrinsic stability to quickly reflect transcriptional dynamics. It is important to have an equal number of cells plated in each testing condition to avoid any incorrect readouts. Reporter assays could be single or dual reporter assays. The reporter could be both luciferases. Most dual-luciferase assays involve adding two reagents to each sample and measuring luminescence following each addition. Adding the first reagent activates the first luciferase reporter reaction; adding the second reagent extinguishes first luciferase reporter activity and initiates the second luciferase reaction. Dual-luciferase assays have some advantages, including 1. reduces variability, 2. reduces background, 3. normalizes differences in transfection efficiencies between samples.

Cellular assays Reporter gene assay β-galactosidase substrates SK-Hep-1

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized A549

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Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Ovaries

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Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29

Products Qiagen FastLane Cell Probe Kit (200)

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