siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using RNeasy Protect Cell Mini Kit (50) to perform RNA isolation / purification Cells - primary human mononuclear cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - vascular smooth muscle cells (VSMC)

Get tips on using EasySep™ Human B Cell Enrichment Kit to perform Cell Isolation B cell

Get tips on using RosetteSep™ Human B Cell Enrichment Cocktail to perform Cell Isolation B cell

Get tips on using EasySep™ Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using MojoSort™ Human Pan Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using Slan (M-DC8)+ Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using MagniSort™ Human pan-Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using Dynabeads™ Untouched™ Human Monocytes Kit to perform Cell Isolation Monocyte