CRISPR Mouse Deletion 3T3-L1

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation ERBB2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Mouse - NIH 3T3

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Mouse - NIH 3T3

Get tips on using ATG5 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Atg5

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Mouse - NIH 3T3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse NIH 3T3

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression Mstn

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression CCR-5