Site Directed Mutagenesis (SDM) Human Deletion SKOV3

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - SKOV3

Get tips on using hCas9 to perform CRISPR Human - Deletion Hsp90α

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion CD38

Get tips on using lentiGuide-Puro to perform CRISPR Human - Deletion CLK1

Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion AXL

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion NGRN

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SST3B

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SST3A