Wound healing assay

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - INS-1 832/12

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - MDA-MB-231

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - mouse bone marrow-derived macrophages

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells