Immunohistochemistry Collagen Type III Goat Mouse

Get tips on using mericon DNA Bacteria Kit (100) to perform DNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using RNeasy Protect Bacteria Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HT1080

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HeLa

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay SJSA-1

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Saos-2

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HEK 293T

Get tips on using miScript II RT Kit (50) to perform cDNA synthesis Tissue