Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human endometrium organoids
Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human pancreatic organoids
Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human esophageal organoids
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Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Human Tenon fibroblasts
Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - MDA-MB-231
Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - Human primary MSCs
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
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