siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 to perform Immunohistochemistry Human - E-Cadherin

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human CD14+ cells

Get tips on using Human CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Human - C-Reactive Protein/CRP

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5 to perform Flow cytometry Anti-bodies Human - CD23

Get tips on using Nitrocef disks to perform Reporter gene assay β-lactamase substrates - HEK 293 & HEK 293T cells

Get tips on using CD171 (L1CAM) Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD171/L1CAM

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells