siRNA / miRNA gene silencing Rat RGC-5

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - RCC

Get tips on using 50 bp DNA Step Ladder to perform DNA Ladder 50 bp

Get tips on using Fluorescent 50 bp DNA Ladder to perform DNA Ladder 50 bp

Get tips on using StepUp 500 bp DNA Ladder to perform DNA Ladder 500 bp

Get tips on using Pan Ras Monoclonal Antibody (Ras10) to perform Western blotting Ras

Get tips on using Mucin 5AC Antibody (45M1): sc-21701 to perform Immunohistochemistry Human - Muc-5AC

Get tips on using E-Gel™ 50 bp DNA Ladder to perform DNA Ladder 50 bp

Get tips on using MUC5AC Antibody / Mucin-5AC [clone 45M1] (V2198) to perform Immunohistochemistry Human - Muc-5AC

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.