Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using Mesenchymal Stem Cell Osteogenic Differentiation Medium to perform Stem cell Differentiation media human umbilical mesenchymal stem cells (hUMSCs) differentiation into osteogenic cells

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human hair follicle dermal papilla cells

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human placental venous endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human placental arterial endothelial cells