ChIP acH4 Canine Human

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Get tips on using BRCA2 rtu elisa kit :: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) RTU ELISA Kit to perform ELISA Mouse - BRCA2

Products MyBioSource.com BRCA2 rtu elisa kit :: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) RTU ELISA Kit

Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21

Products Cell Signaling Technology p21 Waf1/Cip1 (12D1) Rabbit mAb #2947

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type prostate cancer PPC1 cells
DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Products Thermo Fisher Scientific DMEM/F-12

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse gastric cancer organoids

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse gastric cancer organoids

Products STEMCELL technologies IntestiCult™ Organoid Growth Medium (Mouse)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - prostate cancer PPC1 cells

Products Sigma-Aldrich Anti-LC3B antibody produced in rabbit

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - prostate cancer PPC1 cells

Products Sigma-Aldrich Anti-ATG4B antibody produced in rabbit

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)

Products Qiagen RNeasy Plus Mini Kit

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)

Products Thermo Fisher Scientific Gibco™DMEM/F-12

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