siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using Anti-SOX9 antibody produced in rabbit to perform Immunohistochemistry Human - SOX9

Get tips on using Anti-DICER1 antibody produced in rabbit to perform Immunohistochemistry Human - Dicer1

Get tips on using PR Antibody (F-4): sc-166169 to perform Immunohistochemistry Human - PR

Get tips on using Estrogen Receptor alpha Monoclonal Antibody (SP1) to perform Immunohistochemistry Human - ER

Get tips on using CDX-2 (EPR2764Y) Rabbit Monoclonal Antibody to perform Immunohistochemistry Human - CDX2

Get tips on using ChIP-IT® Express Shearing Kits to perform ChIP Human - SW480

Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2

Get tips on using EpiQuik Acetyl-Histone H4 ChIP Kit to perform ChIP Human - HepG2

Get tips on using truChIP Chromatin Shearing Kit with Formaldehyde to perform ChIP Human - T47D

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.