siRNA / RNAi /miRNA transfection Human Cells KG-1

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Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Kupffer Cells

Products Merck Millipore EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human peripheral blood mononuclear cells (PBMCs)

Products Thermo Fisher Scientific Qubit RNA HS Assay Kit

Get tips on using Senescence Cells Histochemical Staining Kit to perform Cell cycle assay mouse - L929

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human trophoblast cells

Products Thermo Fisher Scientific Quant-iT™ RiboGreen™ RNA Assay Kit

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - HOG

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - STTG1

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - Caco-2

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - SH-SY5Y

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates BHK-21 baby hamster kidney cells

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