ChIP acH3 Rat Sheep BEF Tag

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Beclin 1

Get tips on using β Tubulin Antibody (TUB 2.1): sc-58886 to perform Western blotting Tubulin beta

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Cells - immortalized bEnd.3

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Get tips on using PI 3-kinase p100 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B PIK3C3

Get tips on using Monoclonal Anti-Laminin antibody produced in mouse to perform Western blotting Laminin subunit Beta-2

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.