Immunohistochemistry Collagen Type III Goat Mouse

Get tips on using Mouse Adiponectin ELISA Kit (ab108785) to perform ELISA Mouse - Adiponectin

Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Mouse IGF1 ELISA Kit (ab100695) to perform ELISA Mouse - IGF-I

Get tips on using GM-CSF Mouse ELISA Kit to perform ELISA Mouse - GM-CSF

Get tips on using Mouse GM-CSF DuoSet ELISA to perform ELISA Mouse - GM-CSF

Get tips on using Mouse Dkk-1 ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using Mouse BMP2 ELISA Kit (ab119582) to perform ELISA Mouse - BMP-2

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Mouse SDF1 ELISA Kit (ab100741) to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103