Site Directed Mutagenesis (SDM) Human Point mutation HEK293

Get tips on using pPICZ-mEGFP-cmCLC to perform Protein Expression Eukaryotic cells - HEK293 mEGFP-cmCLC

Get tips on using pGEn2-GFP-FcgRIIIa to perform Protein Expression Eukaryotic cells - HEK293 GFP-FcgRIIIa

Get tips on using pHR-CMV-TetO2-CD45 to perform Protein Expression Eukaryotic cells - HEK293 CD45

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HEK293

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pCEP-Pu to perform Protein Expression Eukaryotic cells - HEK293 Guinea Pig TNF-Alpha

Get tips on using psIL-15Rα/Fc to perform Protein Expression Eukaryotic cells - HEK293 sIL-15Rα/Fc

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Immortalized cell lines HEK293

Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HEK293

Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP