The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.
The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood mononuclear cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human lung microvascular endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells
Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment