siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6

Get tips on using pPICZαC-MRP4-his6 to perform Protein Expression Eukaryotic cells - P. pastoris human MRP4-his6

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using CometChip Electrophoresis Starter Kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using Comet SCGE assay kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - bone marrow mononuclear cells

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human chondrocytes

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human podocytes

Get tips on using RediPlate™ 96 RiboGreen™ RNA Quantitation Kit to perform RNA quantification Fuorimetric - human colorectal adenocarcinoma cells (CL-187)