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Get tips on using FastDNA™ SPIN Kit for Feces to perform DNA isolation / purification Bacteria - Gram positive Clostridium difficile

Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform DNA isolation / purification Tissue - murine tail biopsies

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rabbit bone marrow mesenchymal stem cells

Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into chondrogenic cells

Get tips on using Mesenchymal Stem Cell Adipogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into adipogenic cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?