siRNA / miRNA gene silencing Rat IEC-6

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As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Proximal tubular cells (rPT)

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Get tips on using RAC1 Antibody to perform Western blotting Rac1

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Get tips on using Wide-Range DNA Ladder to perform DNA Ladder Long Range

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Get tips on using Medium Range DNA Ruler to perform DNA Ladder Medium Range

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