DNA isolation / purification Cells

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U266

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U266 -

Get tips on using EpiTect Bisulfite Kit to perform Bisulfite DNA Modification Fluids

Get tips on using Investigator Quantiplex HYres Kit to perform DNA quantification Blood

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay SJSA-1

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Saos-2

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HEK 293T

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay COV362

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay COV362