Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using CONFIRM anti-Estrogen Receptor (ER) (SP1) Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Human - ER

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media human umbilical mesenchymal stem cells (hUMSCs) differentiation into osteogenic cells

Get tips on using BrainPhys™ Without Phenol Red to perform Stem cell Differentiation media Differentiation of Human iPSCs into Basal Forebrain cholinergic neurons (BFCN)

Get tips on using Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides to perform Stem cell Differentiation media Human oogonial stem cells differentiation into oocytes

Get tips on using DMEM/Ham's F-12 liquid medium w/o L-Glutamine to perform Stem cell culture media Human Tendon Stem/Pluripotence cells (TSPCs)

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells