Get tips on using Keratinocyte SFM (1X) to perform 3D Cell Culture Media Human prostate organoid
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Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human liver organoids
Get tips on using mTeSR™1 to perform Stem cell culture media Human WA09 hESC
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Get tips on using RNAprotect Cell Reagent (250 ml) to perform RNA stabilization Cell lines
Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Human - Fibroblast cell lines
Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC
Get tips on using Porcine Endothelial Cell Growth Medium to perform Mammalian cell culture media PCAEC
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