TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat brain tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat brain tissue
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat spleen tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat spleen tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat heart tissue
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue
Get tips on using QIAzol Lysis Reagent to perform RNA isolation / purification Tissue - rat lung tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat lung tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat liver tissue
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment