Protein Expression Eukaryotic cells BHK cells

Get tips on using pMH3-rFVII to perform Protein Expression Eukaryotic cells - CHO rFVII

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Get tips on using pBGP3-RsEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-NtEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-G1NkBG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-G1mgNtBG1 to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using pONE-30A to perform Protein Expression Eukaryotic cells - S. frugiperda MBP

Get tips on using AcP(−)p6.9hSEAP to perform Protein Expression Eukaryotic cells - S. frugiperda hSEAP