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Get tips on using pBGP3-G1mgNtBG1 to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Products Manabu Arioka, Department of Biotechnology, The University of To pBGP3-G1mgNtBG1

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Mammalian cells Human eutopic endometrial stromal cells
pONE-30A Product

Get tips on using pONE-30A to perform Protein Expression Eukaryotic cells - S. frugiperda MBP

Products László Beinrohr, Institute of Enzymology, Research Centre for pONE-30A

Get tips on using AcP(−)p6.9hSEAP to perform Protein Expression Eukaryotic cells - S. frugiperda hSEAP

Products Donald L. Jarvis, Department of Molecular Biology, University of AcP(−)p6.9hSEAP

Get tips on using AcP(−)p6.9hEPO to perform Protein Expression Eukaryotic cells - S. frugiperda hEPO

Products Donald L. Jarvis, Department of Molecular Biology, University of AcP(−)p6.9hEPO
pDDGFP-2 Product

Get tips on using pDDGFP-2 to perform Protein Expression Eukaryotic cells - S. cerevisiae GPCRs

Products Mitsunori Shiroish, Graduate School of Pharmaceutical Sciences, pDDGFP-2
pCH1-HRP Product

Get tips on using pCH1-HRP to perform Protein Expression Eukaryotic cells - P. pastoris HRP

Products Geoff P. Lin-Cereghino, Department of Biological Sciences, Unive pCH1-HRP

Get tips on using pPICZαB-CalB to perform Protein Expression Eukaryotic cells - P. pastoris CalB

Products J. Lin-Cereghino, Department of Biological Sciences, University pPICZαB-CalB

Get tips on using pPICZα-TbCLP to perform Protein Expression Eukaryotic cells - P. pastoris TbCLP

Products Justyna Bień-Kalinowska, Witold Stefanski Institute of Parasito pPICZα-TbCLP
pPICZ-B Product

Get tips on using pPICZ-B to perform Protein Expression Eukaryotic cells - P. pastoris TRAAK

Products Roderick MacKinnon, Laboratory of Molecular Neurobiology and Bio pPICZ-B

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