Site Directed Mutagenesis (SDM) Human Point mutation HEK293

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - HEK293

Get tips on using pHR-CMV-TetO2-VSV-G to perform Protein Expression Eukaryotic cells - HEK293 VSV-G

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HEK293

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - HEK293

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HEK293T

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HEK293

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - HEK293

Get tips on using pcDNA™3.1D/V5-His TOPO®-hsEH to perform Protein Expression Eukaryotic cells - HEK293 hsEH

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.