Site Directed Mutagenesis (SDM) Rat Point mutation

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Rat C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103

Get tips on using PE Rat Anti-Mouse CD25 to perform Flow cytometry Anti-bodies Mouse - CD25

Get tips on using PE Rat Anti-Mouse CD49b to perform Flow cytometry Anti-bodies Mouse - CD49b

Get tips on using PE Rat Anti-Mouse CD73 to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using PerCP Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a

Get tips on using FITC Rat Anti-Mouse CD4 to perform Flow cytometry Anti-bodies Mouse - CD4

Get tips on using FITC Rat Anti-Mouse CD74 to perform Flow cytometry Anti-bodies Mouse - CD74

Get tips on using PE Rat anti-Mouse CD34 to perform Flow cytometry Anti-bodies Mouse - CD34