siRNA / miRNA gene silencing Mouse MS1

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MCF-7

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Choroid plexus-like tissue generation from SFEBq

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using PowerPlex® Y23 System to perform Cell line authentication ML14 lymphoblastoid cell line

Get tips on using SNP Trace™ Panel to perform Cell line authentication ML14 lymphoblastoid cell line

Get tips on using PowerPlex® Fusion 6C System to perform Cell line authentication ML14 lymphoblastoid cell line

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - M213

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication ML14 lymphoblastoid cell line

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.