siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line)

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion RNase L

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hESC-EC

Get tips on using Anti-acetyl-Histone H3 Antibody to perform ChIP acH3 - Rabbit Human YFP

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using CC10 Antibody (E-11): sc-365992 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using CC10 Antibody (B-6): sc-390313 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using FGF-10 Antibody (3C7): sc-293208 to perform Immunohistochemistry Human - FGF-10

Get tips on using Mucin 5AC Antibody (45M1): sc-21701 to perform Immunohistochemistry Human - Muc-5AC

Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2