siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells

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Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

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Get tips on using DMEM/F-12 PLUS Basal Medium to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Products Sigma-Aldrich DMEM/F-12 PLUS Basal Medium

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform Stem cell culture media Human Dental pulp stem cells (hDPSC)

Products Thermo Fisher Scientific Gibco™ DMEM, high glucose, GlutaMAX™ Supplement

DNA DNA isolation / purification Cells Immortalized cell lines Loucy

DNA DNA isolation / purification Cells Immortalized cell lines MKN45

Proteins Protein expression and purification Insect cells Sf9 Drosha

Proteins Protein expression and purification Insect cells Hi5 TYR

Proteins Protein expression and purification Insect cells S2 HER2

Proteins Protein expression and purification Mammalian cells HeLa ChaC1

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines HeLa

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