siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

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Cellular assays Cell line authentication Human lung carcinoma cell line NCI-H1299

Get tips on using Gibco™Ham's F-12K (Kaighn's) Medium to perform Stem cell culture media Human myogenic progenitor cells

Products Thermo Fisher Scientific Gibco™Ham's F-12K (Kaighn's) Medium

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Products Sigma-Aldrich Live/Dead Cell Double Staining Kit

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human salivary gland stem cells

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Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human salivary gland stem cells

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Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human intestinal stem cells/organoids

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Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell culture media Human intestinal stem cells/organoids

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling mouse hematopoietic stem cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling mouse primordial germ cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling C2C12 mouse myoblast cells

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