Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HEK 293

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - HEK 293

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - HEK 293

Get tips on using hCas9 to perform CRISPR Human - Deletion Hsp90α

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion CD38

Get tips on using lentiGuide-Puro to perform CRISPR Human - Deletion CLK1

Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion AXL