Get tips on using p-JNK Antibody (G-7): sc-6254 to perform Western blotting JNK
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using HIF-1α Antibody (28b): sc-13515 to perform Western blotting HIF-1 alpha
Get tips on using COL3A1 Antibody (B-10): sc-271249 to perform Western blotting Type III collagen
Get tips on using COL1A Antibody (COL-1): sc-59772 to perform Western blotting Type I collagen
Get tips on using Lamin A/C Monoclonal Antibody (mab636) to perform Western blotting Lamin A/C
Get tips on using Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) to perform Western blotting tubulin gamma
Get tips on using γ Tubulin Antibody (TU-30): sc-51715 to perform Western blotting tubulin gamma
Get tips on using ICAM-1 Antibody (G-5): sc-8439 to perform Western blotting ICAM-1
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