shRNA gene silencing Human Islets of langerhans ZEB1

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Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - A549 & LTEP-a-2 Lipofectamine

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Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Cal 27 cells Polymer / lipid

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Get tips on using pFastBac-GP67-H6HA1-His-RhPV-IRES-EGFP to perform Protein Expression Eukaryotic cells - S. frugiperda HA1 of H6N1 AIV

Products Rong-Huay Juang, Institute of Biotechnology, National Taiwan Uni pFastBac-GP67-H6HA1-His-RhPV-IRES-EGFP

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Discussions Problem in phase separation after using serum/plasma kit

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA methylation profiling Whole genome profiling - human germ cell cancer

Products Epigentek MethylFlash Methylated DNA 5-mC Quantification Kit

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

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Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Products Sigma-Aldrich Trichloroacetic acid

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human osteoblasts - osteoarthritis

Products Thermo Fisher Scientific TRIzol Reagent

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