Site Directed Mutagenesis (SDM) Monkey Deletion COS-7

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa) #13070 to perform Protein Ladder Prestained

Get tips on using Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949 to perform Protein Ladder Prestained

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine coronary artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Get tips on using QIAGEN Large-Construct Kit to perform Plasmid Isolation Acinetobacter calcoaceticus

Get tips on using QIAGEN Large-Construct Kit to perform Plasmid Isolation Pseudomonas aeruginosa