Stem cell culture media

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Nthy-Ori-3

Get tips on using SQSTM1/p62 (D5L7G) Mouse mAb #88588 to perform Autophagy assay cell type - MDA-MB-231

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - Human primary MSCs

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - SK-MEL-28

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - PA-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - MG-63