shRNA gene silencing Human HEK 293T

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Gentra Puregene Cell Kit Plus (6.7 x 109) to perform DNA isolation / purification Cells - Immortalized cell lines H1 hESc

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using pJAP37 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using YFP‐TOP2β to perform Protein Expression Eukaryotic cells - HEK293 TOP2β

Get tips on using YFP‐TOP2α to perform Protein Expression Eukaryotic cells - HEK293 TOP2α

Get tips on using pcDNA-nGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1