ChIP H3K4Me3 Rat

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using ChromaFlash High-Sensitivity ChIP Kit to perform ChIP Rat - Spinal cord

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - INS-1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - Heart

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - H4IIE

Get tips on using Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Liver

Get tips on using Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - Mesenteric arteries