ChIP H3K4Me3 Sheep Rat

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - Colon

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - NRK52E

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Rat - NRK52E

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Liver

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Rat - Pancreas

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Pancreas

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using ChromaFlash High-Sensitivity ChIP Kit to perform ChIP Rat - Spinal cord

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - INS-1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.