ELISA Rat

Get tips on using Rat TNF-alpha Quantikine ELISA Kit to perform ELISA Rat - TNF-alpha

Get tips on using Rat beta NGF ELISA Kit (ab193736) to perform ELISA Rat - beta-NGF

Get tips on using Rat IL-6 Quantikine ELISA Kit to perform ELISA Rat - IL-6

Get tips on using Rat IL-6 ELISA Kit (ab100772) to perform ELISA Rat - IL-6

Get tips on using Rat IGF-1 ELISA Kit (ab213902) to perform ELISA Rat - IGF-I

Get tips on using Rat / Mouse FGF-21 ELISA Kit to perform ELISA Rat - FGF-21

Get tips on using Rat Cytochrome c(CYCS) ELISA kit to perform ELISA Rat - Cytochrome c

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.