ChIP H3K27me3 Rabbit Rat

Get tips on using Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) to perform ChIP Anti-bodies H3K9me3

Get tips on using Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) to perform ChIP Anti-bodies H3K4me3

Get tips on using Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) to perform ChIP Anti-bodies RUNX1

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - Caco-2

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - THP-1

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - HEK 293

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Hepa-1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - Fibroblast cell lines

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.