Immunohistochemistry Anti-mouse IgG Donkey Mouse

Get tips on using Anti-CD3, Clone F7.2.38 to perform Immunohistochemistry CD3 - Mouse Human -NA-

Get tips on using Mouse/Rat Neuropilin-1 Antibody to perform Immunohistochemistry Mouse - Nrp1

Get tips on using Anti-PDHA1 (phospho S293) antibody (ab92696) to perform Immunohistochemistry Mouse - Pyruvate Dehydrogenase

Get tips on using Anti-NF-kB p65 antibody (ab16502) to perform Immunohistochemistry Mouse - NFκB / p65

Get tips on using Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO) to perform Western blotting ZO-1

Get tips on using Anti-UCP-1 antibody produced in rabbit to perform Immunohistochemistry Mouse - UCP1

Get tips on using Anti-CD133 antibody - Stem Cell Marker (ab19898) to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Immunohistochemistry Mouse - p62

Get tips on using Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508) to perform Immunohistochemistry Mouse - Lysozyme

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).