Immunohistochemistry CD3

Get tips on using CD326 (EpCAM) Monoclonal Antibody (G8.8), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM

Get tips on using EasySep™ Human Cord Blood CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Get tips on using CD33 Monoclonal Antibody (WM-53 (WM53)), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD33

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

Get tips on using CD326 (EpCAM) Monoclonal Antibody (G8.8), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM

Get tips on using CD31 (PECAM-1) Monoclonal Antibody (390), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.