Immunohistochemistry CD3 Mouse

Get tips on using APC anti-mouse CD117 (c-kit) Antibody to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using Mouse Prolactin DuoSet ELISA to perform ELISA Mouse - PRL

Get tips on using Mouse Myeloperoxidase DuoSet ELISA to perform ELISA Mouse - MPO

Get tips on using Mouse Leptin ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Mouse Decorin ELISA Kit to perform ELISA Mouse - Decorin

Get tips on using PE Mouse Anti-Human CD140a to perform Flow cytometry Anti-bodies Human - CD140/PDFGR2

Get tips on using PE Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4

Get tips on using APC Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4

Get tips on using BUV395 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).