Immunohistochemistry chk2 phospho (Thr 68) Rabbit IgG Human

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - MUC-6

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-5AC

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-2

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-1

Get tips on using REG1 beta Polyclonal Antibody to perform Immunohistochemistry Human - REG1

Get tips on using Anti-Hes1 antibody (ab49170) to perform Immunohistochemistry Human - Hes1

Get tips on using Anti-Notch1 antibody (ab27526) to perform Immunohistochemistry Human - Notch1

Get tips on using MLH1 antibody [G168-15] to perform Immunohistochemistry Human - MLH1

Get tips on using Anti-CRISP3 antibody (ab105951) to perform Immunohistochemistry Human - CRISP3

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.